Cationic liposomes for generic signal amplification strategies in bioassays

Detection strategies for bioassays based on luminescent lanthanide complexes and signal amplification.

Two attractive detection strategies for bioassays are reviewed in this article. Both approaches use the highly sensitive time-resolved luminescence detection of lanthanide complexes in combination with a signal amplification scheme. While enzyme-amplified lanthanide luminescence (EALL) has been an established technique for more than a decade, nanoparticles doped with luminescent lanthanide comp...

Tyramide Signal Amplification Strategies for Fluorescence Labeling

Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes

PURPOSE Cationic liposomes (CLs) are composed of phospholipid bilayers. One of the most important applications of these particles is in drug and gene delivery. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. The aim of this study was to develop novel CLs containing magnetite to overcome the defi...

Cationic Liposomes in Different Structural Levels for Gene Delivery

Over the last few decades, as a promising strategy for the treatment of many refractory diseases, such as inherited diseases (Martin-Rendon & Blake, 2003) and acquired immunodeficiency syndrome (AIDS) (Fanning et al., 2003), gene therapy, the objective to allow a gene to express the protein coded in the target cells and consequently to treat disease by the protein secreted from cells transfecte...

Targeted Cationic Liposomes , Technologies , and Developments

Anas El-Aneed is a doctoral student in biochemistry at the School of Pharmacy at Memorial University of Newfoundland, 300 Prince Philip Dr., St. John’s, NL, A1B 3V6 Canada, tel. 709.737.4331, fax 709.777.7044, [email protected]. ationic liposomes are widely used to transfer genetic materials into specific cells. A good liposomal formulation for gene therapy should encapsulate and protect the nu...